LOCUS       pDONR™/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(1877..1973)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(1988..2007)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2015..2031
                     /note="M13 Reverse priming site"
     gene            2144..2953
                     /note="Kanamycin resistance gene"
     rep_origin      3074..3747
                     /note="pUC origin"
     vector          join(1885..3750,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..1870
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:31814 IMAGE:4137487"
                     /tissue_type="Muscle, rhabdomyosarcoma"
                     /clone_lib="NIH_MGC_17"
                     /lab_host="DH10B-R"
                     /note="Vector: pOTB7"
     gene            692..1870
                     /gene="CHST8"
                     /db_xref="GeneID:64377"
                     /db_xref="HGNC:15993"
                     /db_xref="MIM:610190"
     CDS             692..1870
                     /dnas_title="carbohydrate (N-acetylgalactosamine 4-0)
                     sulfotransferase 8"
                     /gene="CHST8"
                     /codon_start=1
                     /product="carbohydrate (N-acetylgalactosamine 4-0)
                     sulfotransferase 8"
                     /protein_id="AAH18723.1"
                     /db_xref="GI:19387972"
                     /db_xref="GeneID:64377"
                     /db_xref="HGNC:15993"
                     /db_xref="MIM:610190"
                     /translation="MTLRPGTMRLACMFSSILLFGAAGLLLFISLQDPTELAPQQVPG
                     IKFNIRPRQPHHDLPPGGSQDGDLKEPTERVTRDLSSGAPRGRNLPAPDQPQPPLQRG
                     TRLRLRQRRRRLLIKKMPAAATIPANSSDAPFIRPGPGTLDGRWVSLHRSQQERKRVM
                     QEACAKYRASSSRRAVTPRHVSRIFVEDRHRVLYCEVPKAGCSNWKRVLMVLAGLASS
                     TADIQHNTVHYGSALKRLDTFDRQGILHRLSTYTKMLFVREPFERLVSAFRDKFEHPN
                     SYYHPVFGKAILARYRANASREALRTGSGVRFPEFVQYLLDVHRPVGMDIHWDHVSRL
                     CSPCLIDYDFVGKFESMEDDANFFLSLIRAPRNLTFPRFKDRHSQEARTTARIAHQYF
                     AQLSALQRQRTYDFYYMDYLMFNYSKPFADLY"
     misc_feature    692..1870
                     /note="CHST8 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg ccaggaccct acggagctcg ccccccagca
        721 ggtgccagga ataaagttca acatcaggcc aaggcagccc caccacgacc tcccaccagg
        781 cggctcccag gatggtgact tgaaggaacc cacagagagg gtcactcggg acttatccag
        841 tggggccccg aggggccgca acctgccagc gcctgaccag cctcaacccc cgctgcagag
        901 gggaacccgt ctgcggctcc gccagcgccg tcgccgtctg ctcatcaaga aaatgccagc
        961 tgcggcgacc atcccggcca acagctcgga cgcgcccttc atccggccgg gacccgggac
       1021 gctggatggc cgctgggtca gcctgcaccg gagccagcag gagcgcaagc gggtgatgca
       1081 ggaggcctgc gccaagtacc gggcgagcag cagccgccgg gccgtcacgc cccgccacgt
       1141 gtcccgtatc ttcgtggagg accgccaccg cgtgctctac tgcgaggtgc ccaaggccgg
       1201 ctgctccaat tggaagcggg tgctcatggt gctggccggc ctggcctcgt ccactgccga
       1261 catccagcac aacaccgtcc actatggcag cgctctcaag cgcctggaca ccttcgaccg
       1321 ccagggtatc ttgcaccgtc tcagcaccta caccaagatg ctctttgtcc gcgagccctt
       1381 cgagaggctg gtgtccgcct tccgcgacaa gtttgagcac cccaacagct actatcaccc
       1441 ggtcttcggc aaggccatcc tggcccggta ccgcgccaat gcctctcggg aggccctgcg
       1501 gaccggctct ggggtgcgtt ttcccgagtt cgtccagtac ctgctggacg tgcaccggcc
       1561 cgtggggatg gacattcact gggaccatgt cagccggctc tgcagcccct gcctcatcga
       1621 ctacgatttc gtaggcaagt tcgagagcat ggaggacgat gccaacttct tcctgagcct
       1681 catccgcgcg ccgcggaacc tgaccttccc ccggttcaag gaccggcact cgcaggaggc
       1741 gcggaccaca gcgaggatcg cccaccagta cttcgcccaa ctctcggccc tgcaaaggca
       1801 gcgcacctac gacttctact acatggatta cctgatgttc aactattcca agccctttgc
       1861 agatctgtac TAGGACCCAG CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT
       1921 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTTGCCATCC
       1981 AGCTGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG
       2041 GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT AAAATAATAT CATCATGAAC
       2101 AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT GTTATGAGCC ATATTCAACG
       2161 GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT GATTTATATG GGTATAAATG
       2221 GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT CGCTTGTATG GGAAGCCCGA
       2281 TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT GCCAATGATG TTACAGATGA
       2341 GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT CCGACCATCA AGCATTTTAT
       2401 CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC CCCGGAAAAA CAGCATTCCA
       2461 GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT GATGCGCTGG CAGTGTTCCT
       2521 GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT AACAGCGATC GCGTATTTCG
       2581 TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT GATGCGAGTG ATTTTGATGA
       2641 CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA ATGCATAAAC TTTTGCCATT
       2701 CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT GATAACCTTA TTTTTGACGA
       2761 GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA ATCGCAGACC GATACCAGGA
       2821 TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT TCATTACAGA AACGGCTTTT
       2881 TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG CAGTTTCATT TGATGCTCGA
       2941 TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC TGGCAGAGCA TTACGCTGAC
       3001 TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC GTGAGTTACG CGTCGTTCCA
       3061 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
       3121 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
       3181 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
       3241 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
       3301 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
       3361 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
       3421 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
       3481 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
       3541 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
       3601 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
       3661 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
       3721 GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//